Zinc tetraruthenated porphyrin binding and photoinduced oxidation of calf-thymus DNA.

نویسندگان

  • K Araki
  • C A Silva
  • H E Toma
  • L H Catalani
  • M H Medeiros
  • P Di Mascio
چکیده

The photooxidation of calf-thymus DNA has been investigated in the presence of a supramolecular tetraruthenated zincporphyrin (ZnTRP) sensitizer. A strong interaction of ZnTRP with DNA has been observed, exhibiting a gradual transition from a non-specific electrostatic binding mode to a more specific one at high DNA concentrations. Formation of O2(1delta(g)) has been detected from its near-infrared emission, after the excitation of ZnTRP in dioxygen-containing solutions. In the presence of DNA and dioxygen, ZnTRP promotes efficient photocatalytic oxidation of the 2'-deoxyguanosine sites, via their direct reaction with O2(1delta(g)), as in a previous work on the ZnTRP-photoinduced oxidation of the free nucleosides.

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عنوان ژورنال:
  • Journal of inorganic biochemistry

دوره 78 4  شماره 

صفحات  -

تاریخ انتشار 2000